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Protease supplementation improves muscle function after eccentric exercise.
Med Sci Sports Exerc. 2009 Oct; 41(10):1908-14.MS

Abstract

Protease supplementation has been purported to reduce the damaging effects of eccentric exercise and accelerate recovery of muscle function, possibly by regulating inflammation.

PURPOSE

To determine the effectiveness of protease supplementation in attenuating eccentric exercise-induced skeletal muscle damage and inflammation.

METHODS

After standard physical and hemodynamic assessment and fasting venous blood samples, subjects performed isokinetic extension/flexion of the quadriceps group on a Biodex isokinetic dynamometer at 60°·s(-1), followed by VO2max testing. Subjects were randomly assigned to consume 5.83 g daily of either a cellulose placebo (N = 15; 22.27 ± 3.33 yr, 71.17 ± 2.91 inches, 179.4 ± 24.05 lb, 50.55 ± 5.66 mL·kg(-1)·min(-1)) or a proteolytic supplement containing fungal proteases, bromelain, and papain (N = 14; 22.85 ± 5.9 yr, 70.0 ± 2.67 inches, 173.11 ± 29.94 lb, 49.69 ± 6.15 mL·kg(-1)·min(-1)) for a period of 21 d. After the supplementation period, subjects donated blood samples before performing a 45-min downhill (-17.5%) treadmill protocol at 60% of VO2max. An additional four blood draws and three muscle function tests were performed during the next 48 h. Blood was analyzed using standard hematology and clinical chemistry, enzyme-linked immunosorbent assay, and bead array. Blood data were analyzed using multivariate analysis of variance (MANOVA) with repeated measures, whereas Biodex data were analyzed using a MANOVA on %Δ values.

RESULTS

Significant group differences (T1-T3, P = 0.033; T1-T4, P = 0.043) and another strong trend (T1-3 h, P = 0.055) were observed for flexion (peak torque %Δ at 60°·s(-1)) indicating higher force production in the protease group. Significant group × time interactions (P < 0.05) were observed, including elevations in circulating eosinophils and basophils in the protease group coinciding with lower levels of serum cyclooxygenase 2, interleukin 6, and interleukin 12 in this group.

CONCLUSIONS

Protease supplementation seems to attenuate muscle strength losses after eccentric exercise by regulating leukocyte activity and inflammation.

Authors+Show Affiliations

Exercise and Biochemical Nutrition Laboratory, Baylor University, Waco, TX 76798, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19727022

Citation

Buford, Thomas W., et al. "Protease Supplementation Improves Muscle Function After Eccentric Exercise." Medicine and Science in Sports and Exercise, vol. 41, no. 10, 2009, pp. 1908-14.
Buford TW, Cooke MB, Redd LL, et al. Protease supplementation improves muscle function after eccentric exercise. Med Sci Sports Exerc. 2009;41(10):1908-14.
Buford, T. W., Cooke, M. B., Redd, L. L., Hudson, G. M., Shelmadine, B. D., & Willoughby, D. S. (2009). Protease supplementation improves muscle function after eccentric exercise. Medicine and Science in Sports and Exercise, 41(10), 1908-14. https://doi.org/10.1249/MSS.0b013e3181a518f0
Buford TW, et al. Protease Supplementation Improves Muscle Function After Eccentric Exercise. Med Sci Sports Exerc. 2009;41(10):1908-14. PubMed PMID: 19727022.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protease supplementation improves muscle function after eccentric exercise. AU - Buford,Thomas W, AU - Cooke,Matthew B, AU - Redd,Liz L, AU - Hudson,Geoffrey M, AU - Shelmadine,Brian D, AU - Willoughby,Darryn S, PY - 2009/9/4/entrez PY - 2009/9/4/pubmed PY - 2011/11/9/medline SP - 1908 EP - 14 JF - Medicine and science in sports and exercise JO - Med Sci Sports Exerc VL - 41 IS - 10 N2 - UNLABELLED: Protease supplementation has been purported to reduce the damaging effects of eccentric exercise and accelerate recovery of muscle function, possibly by regulating inflammation. PURPOSE: To determine the effectiveness of protease supplementation in attenuating eccentric exercise-induced skeletal muscle damage and inflammation. METHODS: After standard physical and hemodynamic assessment and fasting venous blood samples, subjects performed isokinetic extension/flexion of the quadriceps group on a Biodex isokinetic dynamometer at 60°·s(-1), followed by VO2max testing. Subjects were randomly assigned to consume 5.83 g daily of either a cellulose placebo (N = 15; 22.27 ± 3.33 yr, 71.17 ± 2.91 inches, 179.4 ± 24.05 lb, 50.55 ± 5.66 mL·kg(-1)·min(-1)) or a proteolytic supplement containing fungal proteases, bromelain, and papain (N = 14; 22.85 ± 5.9 yr, 70.0 ± 2.67 inches, 173.11 ± 29.94 lb, 49.69 ± 6.15 mL·kg(-1)·min(-1)) for a period of 21 d. After the supplementation period, subjects donated blood samples before performing a 45-min downhill (-17.5%) treadmill protocol at 60% of VO2max. An additional four blood draws and three muscle function tests were performed during the next 48 h. Blood was analyzed using standard hematology and clinical chemistry, enzyme-linked immunosorbent assay, and bead array. Blood data were analyzed using multivariate analysis of variance (MANOVA) with repeated measures, whereas Biodex data were analyzed using a MANOVA on %Δ values. RESULTS: Significant group differences (T1-T3, P = 0.033; T1-T4, P = 0.043) and another strong trend (T1-3 h, P = 0.055) were observed for flexion (peak torque %Δ at 60°·s(-1)) indicating higher force production in the protease group. Significant group × time interactions (P < 0.05) were observed, including elevations in circulating eosinophils and basophils in the protease group coinciding with lower levels of serum cyclooxygenase 2, interleukin 6, and interleukin 12 in this group. CONCLUSIONS: Protease supplementation seems to attenuate muscle strength losses after eccentric exercise by regulating leukocyte activity and inflammation. SN - 1530-0315 UR - https://wwww.unboundmedicine.com/medline/citation/19727022/Protease_supplementation_improves_muscle_function_after_eccentric_exercise_ L2 - https://doi.org/10.1249/MSS.0b013e3181a518f0 DB - PRIME DP - Unbound Medicine ER -