Tags

Type your tag names separated by a space and hit enter

The effects of colchicine on a Peyronie's-like condition in an animal model.
J Urol. 1999 Jun; 161(6):1980-3.JU

Abstract

PURPOSE

We have developed an animal model of Peyronie's disease by injecting transforming growth factor beta (TGF-beta) into the rat penis. Our objective is to study the effects of colchicine on the Peyronie's condition in an animal model.

MATERIALS AND METHODS

Thirty-six adult male Sprague-Dawley rats received TGF-beta injections into the tunica albuginea and were divided into two groups (n = 18 each). Rats in the first group were divided into three subgroups (n = 6 each). Each rat in the three subgroups received the following: Subgroup 1 received colchicine, subgroup 2 received ibuprofen, and subgroup 3 received regular water. The rats were euthanized after 6 weeks. Rats in the second group were also divided into three subgroups. These rats received the same treatments as the rats in the first group, but treatments began 6 weeks after TGF-beta injection. These rats were euthanized after 12 weeks. Tunical tissue samples were collected and examined using Hart and trichrome stains, electron microscopy (EM), and western blot analysis for TGF-beta detection.

RESULTS

In the first group, the colchicine-treated rats exhibited less collagen deposition and less elastic fiber fragmentation than the untreated or ibuprofen-treated rats. EM confirmed the results and showed normal distribution and shape of both collagen and elastic fibers in the colchicine-treated group. In the second group, the colchicine-treated rats exhibited less crowding of the collagen fibers. However, the elastic fibers remained fragmented and scarce. Western blot analysis showed significant down-regulation of TGF-beta expression (5/6) in the colchicine-treated group after 6 weeks. Down-regulation was observed in only 1/6 in both ibuprofen and non-treated groups. After 12 weeks 2/6, 1/6, and 1/6 rats displayed down regulation in the colchicine treated, ibuprofen treated, and non-treated groups, respectively.

CONCLUSION

Early colchicine treatment may suppress a Peyronie's like condition in the rat animal model.

Authors+Show Affiliations

Department of Urology, University of California School of Medicine, San Francisco 94143-0738, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10332485

Citation

El-Sakka, A I., et al. "The Effects of Colchicine On a Peyronie's-like Condition in an Animal Model." The Journal of Urology, vol. 161, no. 6, 1999, pp. 1980-3.
El-Sakka AI, Bakircioglu ME, Bhatnagar RS, et al. The effects of colchicine on a Peyronie's-like condition in an animal model. J Urol. 1999;161(6):1980-3.
El-Sakka, A. I., Bakircioglu, M. E., Bhatnagar, R. S., Yen, T. S., Dahiya, R., & Lue, T. F. (1999). The effects of colchicine on a Peyronie's-like condition in an animal model. The Journal of Urology, 161(6), 1980-3.
El-Sakka AI, et al. The Effects of Colchicine On a Peyronie's-like Condition in an Animal Model. J Urol. 1999;161(6):1980-3. PubMed PMID: 10332485.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of colchicine on a Peyronie's-like condition in an animal model. AU - El-Sakka,A I, AU - Bakircioglu,M E, AU - Bhatnagar,R S, AU - Yen,T S, AU - Dahiya,R, AU - Lue,T F, PY - 1999/5/20/pubmed PY - 1999/5/20/medline PY - 1999/5/20/entrez SP - 1980 EP - 3 JF - The Journal of urology JO - J Urol VL - 161 IS - 6 N2 - PURPOSE: We have developed an animal model of Peyronie's disease by injecting transforming growth factor beta (TGF-beta) into the rat penis. Our objective is to study the effects of colchicine on the Peyronie's condition in an animal model. MATERIALS AND METHODS: Thirty-six adult male Sprague-Dawley rats received TGF-beta injections into the tunica albuginea and were divided into two groups (n = 18 each). Rats in the first group were divided into three subgroups (n = 6 each). Each rat in the three subgroups received the following: Subgroup 1 received colchicine, subgroup 2 received ibuprofen, and subgroup 3 received regular water. The rats were euthanized after 6 weeks. Rats in the second group were also divided into three subgroups. These rats received the same treatments as the rats in the first group, but treatments began 6 weeks after TGF-beta injection. These rats were euthanized after 12 weeks. Tunical tissue samples were collected and examined using Hart and trichrome stains, electron microscopy (EM), and western blot analysis for TGF-beta detection. RESULTS: In the first group, the colchicine-treated rats exhibited less collagen deposition and less elastic fiber fragmentation than the untreated or ibuprofen-treated rats. EM confirmed the results and showed normal distribution and shape of both collagen and elastic fibers in the colchicine-treated group. In the second group, the colchicine-treated rats exhibited less crowding of the collagen fibers. However, the elastic fibers remained fragmented and scarce. Western blot analysis showed significant down-regulation of TGF-beta expression (5/6) in the colchicine-treated group after 6 weeks. Down-regulation was observed in only 1/6 in both ibuprofen and non-treated groups. After 12 weeks 2/6, 1/6, and 1/6 rats displayed down regulation in the colchicine treated, ibuprofen treated, and non-treated groups, respectively. CONCLUSION: Early colchicine treatment may suppress a Peyronie's like condition in the rat animal model. SN - 0022-5347 UR - https://wwww.unboundmedicine.com/medline/citation/10332485/The_effects_of_colchicine_on_a_Peyronie's_like_condition_in_an_animal_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-5347(05)68868-4 DB - PRIME DP - Unbound Medicine ER -