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- May maternal anti mullerian hormone levels predict adverse maternal and perinatal outcomes in preeclampsia? [JOURNAL ARTICLE]
- J Matern Fetal Neonatal Med 2014 Aug 18.:1-18.
Abstract Background: Prediction of preeclampsia and adverse maternal and perinatal outcomes with biomarkers has been proposed previously. AMH is a growth factor which is primarily responsible of the regression of the mullerian duct, but also used to predict ovarian reserve and decreases with age similar to the fertility. Aim: To evaluate the predictive role of maternal anti mullerian hormone (mAMH) in adverse maternal and perinatal outcomes in preeclampsia. Methods: This prospective case-control study was conducted at current high risk pregnancy department in a tertiary research hospital and 45 cases with preeclampsia classified as study group and 42 as control group. Data collected and evaluated were; age, BMI (body mass index), MD (marriage duration), GW (gestational weeks), gravidity, parity, mode of delivery, birth weight, newborn Apgar score, newborn gender, maternal complication, perinatal outcome, some laboratory parameters and mAMH. The association between mAMH levels and maternal and fetal outcomes were evaluated. Results: There were no statistically significant differences between groups in terms of age, BMI, MD, gravidity, parity and newborn gender (p>0,05). GW, vaginal delivery, birth weight, newborn Apgar score, were statistically significantly lower in preeclamptic patients when compared with nonpreeclamptic patients (p<0,001). Adverse maternal and perinatal outcomes were statistically significantly higher in study group (p<0,001). The laboratory values (ALT, AST, BUN, creatinin, LDH, uric acid and fibrinogen) were statistically significantly lower in control group (p<0,001). The mAMH level was significantly lower in preeclamptic group (p:0,035). There was no correlation between mAMH levels and demographic and clinical parameters. The area under the ROC curve (AUC) was 0.590 and cut off value was 0.225 ng/ml with sensitivity of 88.1 % and specificity of 84.4 % for mAMH. Logistic Regression analysis showed a statistically insignificance between mAMH and maternal complication and perinatal outcome (p:0,249). Conclusion: According to this study, mAMH level was lower in preeclamptic patients than normal pregnants, and found to be a discriminative factor with low sensitivity and specificity. There was no relationship between mAMH and adverse maternal and perinatal outcomes. Further randomized controlled studies with more participants are needed to evaluate the accurate effects of mAMH levels on preeclampsia and should increase the power of mAMH levels in predicting the preeclampsia.
- Safety and efficacy of balloon-occluded transcatheter arterial chemoembolization using miriplatin for hepatocellular carcinoma. [JOURNAL ARTICLE]
- Hepatol Res 2014 Aug 8.
Balloon-occluded transcatheter arterial chemoembolization (B-TACE) using a microballoon catheter was performed to administer miriplatin, and the early therapeutic efficacy and safety of the procedure were evaluated.Out of 158 patients who received miriplatin using B-TACE for hepatocellular carcinoma, 49 patients with a single lesion at either stage I or II (according to the Liver Cancer Study Group of Japan) were evaluated in comparison with 48 matched patients who received miriplatin using conventional-TACE (C-TACE).The mean total dose and median dose of miriplatin in each group were 32.5 ± 31.7 mg and 20 mg (C-TACE) and 50.1 ± 31.3 mg and 40 mg (B-TACE), respectively, (P < 0.01). The treatment effect (TE) on the target nodule classified as TE4, TE3, TE2, or TE1, was 41.4%, 32.8%, 24.1%, and 1.7%, respectively, in the C-TACE group, and 55.1%, 38.8%, 4.1%, and 2.0%, respectively, in the B-TACE group. Therefore, the TE was significantly higher in the B-TACE group (P < 0.05). Although abdominal blood tests revealed adverse, increased levels of serum alanine aminotransferase (ALT) in a significantly higher number of B-TACE treated patients, serum ALT levels returned to baseline levels in all patients within 1 month. There were no significant differences in clinical symptoms between the two groups.Compared to C-TACE, B-TACE significantly improved cancer nodule control, and it was satisfactory in terms of safety. B-TACE is an effective procedure that enhances the effects of catheterization with miriplatin.
- Magnetic Ring Anastomosis of Suprahepatic Vena Cava-novel technique for liver transplantation in rat. [JOURNAL ARTICLE]
- Transpl Int 2014 Jul 31.
To improve the technique of suprahepatic vena cava (SHVC) reconstruction in rat OLT, novel magnetic rings were designed and manufactured to facilitate reconstruction of SHVC and shorten the anhepatic time.120 adult male Wistar rats were randomly divided into 2 groups: Rings group (n=30), using magnetic rings for SHVC reconstruction; Suture group (n=30), 7/0 Prolene suture was used for SHVC running anastomosis as control. Cuff techniques were used for portal vein and infrahepatic vena cava reconstruction as Kamada and Calne described. The bile duct was re-connected with a stent. The hepatic re-arterialization was omitted.In the rings group, the SHVC reconstruction took 0.91 ± 0.24 (mean ± SD) min; the anhepatic phase and the recipient operation time were 5.63 ± 0.65min and 36.02± 8.02 min, respectively. In Suture group, the anastomotic time of SHVC was 10.40±2.11 min; the anhepatic phase and the recipient operation time were 17.76±2.51 and 49.38±12.06 min, respectively, there was statistically significant difference between the two groups. The ALT levels reached peak at 24h post-OLT (186.2 ± 32.5 IU/L) and restored to normal level at 96h gradually. In the rings group, 29 of 30 rats survived at day 7, 28 of 30 rats survived at day 30. In contrast, only 25 of 30 recipients in suture group remained alive at day 7, 22 of 30 remained alive at day 30 (p < 0.05). Better anastomotic healing was founded in rings group by pathology and scanning electron microscope.The magnetic rings technique provides a novel, simple method for SHVC reconstruction of OLT in rat. It significantly shortens anhepatic phase, while the success rate of the operation is satisfactory. This article is protected by copyright. All rights reserved.
- Comparison of Chromogenic In Situ Hybridization and Fluorescence In Situ Hybridization for the Evaluation of MDM2 Amplification in Adipocytic Tumors. [JOURNAL ARTICLE]
- J Clin Lab Anal 2014 Aug 17.
Atypical lipomatous tumor/well-differentiated liposarcoma (ALT-WDLPS) and dedifferentiated liposarcoma (DDLPS) are characterized cytogenetically by a 12q13-15 amplification involving the mouse double minute 2 (MDM2) oncogene. Fluorescence in situ hybridization (FISH) is used frequently to detect this amplification and aid with the diagnosis of these entities, which is difficult by morphology alone. Recently, bright-field in situ hybridization techniques such as chromogenic in situ hybridization (CISH) have been introduced for the determination of MDM2 amplification status.The present study compared the results of FISH and CISH for detecting MDM2 amplification in 41 cases of adipocytic tumors. Amplification was defined in both techniques as a MDM2/CEN12 ratio of 2 or greater.Eleven cases showed amplification with both FISH and CISH, and 26 cases showed no amplification with both methods. Two cases had discordant results between CISH and FISH, and two cases were not interpretable by CISH.CISH is advantageous for allowing pathologists to evaluate the histologic and molecular alterations occurring simultaneously in a specimen. Moreover, CISH is found to be more cost- and time-efficient when used with automation, and the signals do not quench over time. CISH technique is a reliable alternative to FISH in the evaluation of adipocytic tumors for MDM2 amplification.
- Serum hepatitis B virus RNA levels as an early predictor of HBeAg seroconversion during treatment with polymerase inhibitors. [JOURNAL ARTICLE]
- Hepatology 2014 Aug 18.
Background and aims: Hepatitis B e antigen (HBeAg) seroconversion represents an endpoint of treatment of chronic hepatitis B virus (HBV) infections. We have studied whether levels of serum HBV RNA during polymerase inhibitor treatment might be helpful for predicting HBeAg seroconversion. Methods: HBV RNA levels were determined in serial serum samples from 62 patients with chronic HBV infection (50 HBeAg-positive). Patients received antiviral treatment for a mean duration of 30±15 [range 4-64] months. A new RACE-based real-time PCR was established for quantitative analysis of polyadenylated full length (fl) and truncated (tr) HBV RNA. HBV RNA, HBV DNA and HBsAg levels as well as presence of HBeAg and anti-HBe were measured at baseline, month 3, month 6, and subsequent time points. Results: Fifteen patients who achieved HBeAg seroconversion after a mean duration of 19±14 [range 3-56] months of antiviral treatment showed a significantly stronger decline in mean HBV flRNA and trRNA levels from baseline to month 3 of 1.0±1.4 (range, [-1.6] - 3.4) and 2.1±1.4 (range, 0 - 3.9) and to month 6 of 1.8±1.4 (range, 0 - 4.6) and 3.1±1.7 (range, 0 - 5.1) log10 copies/mL, respectively, in comparison to 35 HBeAg-positive patients without HBeAg seroconversion (p<0.001 for months three and six). A similar decline in HBV RNA levels was observed in HBeAg-negative patients. The decline of HBV RNA levels at months 3 and 6 of treatment was to be the strongest predictor of HBeAg seroconversion when compared to levels of HBV DNA, HBsAg, ALT, and to HBV genotype, age and sex. Conclusion: Serum HBV RNA levels may serve as a novel tool for prediction of serologic response during polymerase inhibitor treatment in HBeAg-positive patients. (Hepatology 2014).
- Serum microRNA-124 is a novel biomarker for liver necroinflammation in patients with chronic hepatitis B virus infection. [JOURNAL ARTICLE]
- J Viral Hepat 2014 Aug 8.
Patients with chronic hepatitis B virus (HBV) infection and normal or mildly increased transaminases may have sustained significant liver damage, as verified by liver biopsy. However, no suitable noninvasive method exists for identifying liver necroinflammation in such patients. We aimed to investigate the power of microRNA-124 as a novel biomarker for liver necroinflammation. A total of 131 recruited patients with chronic HBV infection underwent liver biopsy for grading of necroinflammation (G) and staging of fibrosis (S). Thirty healthy individuals were included as controls (HCs). Serum microRNA-124 and microRNA-122 levels were measured using qRT-PCR. Forty-five patients from the study population receiving entecavir therapy were monitored for changes in serum microRNA-124 levels in association with improved liver histology. The capacity of serum microRNA-124 levels in discriminating the grade of liver necroinflammation was compared with alanine aminotransferase (ALT) with liver biopsy validation. Serum microRNA-124 levels were significantly higher in patients with chronic HBV infection than in HCs (P < 0.0001). Patients with considerable liver necroinflammation (G ≥ 2) had significantly higher serum miRNA-124 levels than those without or with mild necroinflammation (P < 0.0001). After 48 weeks of antiviral therapy, serum microRNA-124 levels considerably declined in 45 patients (P < 0.0001), which were associated with histological improvement. In patients with normal ALT and a serum HBV DNA load >10(4) copies/mL, receiver operating characteristic (ROC) curve of serum microRNA-124 levels yielded an area under ROC curve (AUC) of 0.840, with 58.3% sensitivity and 91.7% specificity in discriminating between moderate-to-severe liver necroinflammation (G ≥ 2).
- APRI and FIB-4 are good predictors of the stage of liver fibrosis in chronic hepatitis B: the Chronic Hepatitis Cohort Study (CHeCS). [JOURNAL ARTICLE]
- J Viral Hepat 2014 Aug 1.
We aim to determine the predictive ability of APRI, FIB-4 and AST/ALT ratio for staging of liver fibrosis and to differentiate significant fibrosis (F2-F4) from none to minimal fibrosis (F0-F1) in chronic hepatitis B (CHB). Liver biopsy results were mapped to an F0-4 equivalent fibrosis stage. Mean APRI and FIB-4 scores were significantly higher for each successive fibrosis level from F1 to F4 (P < 0.05). Based on optimized cut-offs, the AUROCs in distinguishing F2-F4 from F0 to F1 were 0.81 (0.76-0.87) for APRI, 0.81 (0.75-0.86) for FIB-4 and 0.56 (0.49-0.64) for AST/ALT ratio. APRI and FIB-4 distinguished F2-F4 from F0 to F1 with good sensitivity and specificity and can be useful for treatment decisions and monitoring progression of fibrosis.
- Response to Dr. Sertoglu and Colleagues. [LETTER]
- Liver Int 2014 Aug 11.
We recently demonstrated that the combination of liver stiffness measurement with NAFLD fibrosis score (NFS), two complementary, easy-to-perform, and widely available tools, is able to accurately diagnose or exclude the presence of severe liver fibrosis, in addition to reducing the number of needed diagnostic liver biopsies by about 50% - 60% . We thank Dr. Sertoglu and colleagues for their comments, which prompted us to further clarify the results of our analyses. First, they pointed out that data from Sicilian and northern Italian patients should be assessed for normal distribution before comparing these patients in terms of demographic, laboratory, metabolic, and histologic variables. In response, we examined the data, and found that only some variables, such as ALT levels, did not have normal distribution This article is protected by copyright. All rights reserved.
- "Celtic migrations": Fact or fiction? Strontium and oxygen isotope analysis of the Czech cemeteries of Radovesice and Kutná Hora in Bohemia. [JOURNAL ARTICLE]
- Am J Phys Anthropol 2014 Aug 18.
Strontium and oxygen isotope analysis of human remains from the early La Tène (fourth/third century BC) Czech cemeteries of Radovesice I (RAD I), Radovesice II (RAD II), and Kutná Hora were conducted to investigate the importance of residential changes during the period of the historic "Celtic migrations". In the initial phases (LT A/B), the grave goods of these cemeteries are typical for the core area of the La Tène culture, while around 300 BC (LT B2) an alteration occurs and typical Bohemian styles arise, and connections to Moravia and the Danubian region become visible. The strontium isotope ratios are highly varied with (87) Sr/(86) Sr values between 0.7062 and 0.7153 in Radovesice, and between 0.7082 and 0.7147 in Kutná Hora. The oxygen isotope data are more homogeneous and yield δ(18) Op ratios from 14.8‰ to 17.2‰ [mean: 16.2‰ ± 0.5 (1σ)] in Radovesice, and from 14.9‰ to 17.3‰ [mean: 16.5‰ ± 0.6 (1σ)] in Kutná Hora. Because the geological properties of the landscapes around the sites are variable and complex, most of the observed variations among the strontium isotope ratios may have been caused by agricultural practices, such as regularly changing farming land. Nevertheless, there are some individuals who differ completely from the regional isotopic baseline values. This suggests that at least a small part of the community migrated, which does not seem to be correlated with any particular phase of the La Tène period. Remarkably, it is mainly males who seem to be of nonlocal origin, and particularly those who were buried as warriors. Females, on the other hand, appear to have been more closely bonded to the Bohemian region. Whether the "foreign" individuals with differing isotopic compositions came from Moravia or the Danubian region remains debatable. Am J Phys Anthropol, 2014. © 2014 Wiley Periodicals, Inc.
- A rhodium coordinated poly(arylene-ethynylene)-alt-poly(arylene-vinylene) copolymer acting as photocatalyst for visible-light powered NAD+/ NADH reduction. [JOURNAL ARTICLE]
- J Am Chem Soc 2014 Aug 18.
A 2,2´-bipyridyl-containing poly(arylene-ethynylene)-alt-poly(arylene-vinylene) polymer acting as a light harvesting ligand system was synthesized and coupled to an organometallic rhodium complex designed for photocatalytic NAD+/NADH reduction. The wide spectral range absorbing material was characterized using various analytical techniques confirming its chemical structure and properties. The dielectric function of the material was determined from spectroscopic ellipsometry measurements. Photocatalytic reduction of nucleotide redox cofactors under visible light irradiation (390 to 650 nm) was performed and discussed in detail. The new metal-containing polymer can be used to cover large surface areas (e.g. glass beads) and, due to this immobilization step, can be easily separated from the reaction solution after photolysis. Because of its high stability, the polymer-based catalyst system can be repeatedly used under different reaction conditions for (photo)chemical reduction of NAD+. With this concept enzymatic, photo-biocatalytic systems for solar energy conversion can be facilitated and the precious metal catalyst can be recycled.