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Human adipose tissue-derived mesenchymal stromal cells promote B-cell motility and chemoattraction.

Abstract

BACKGROUND AIMS

Mesenchymal stromal cells hold special interest for cell-based therapy because of their tissue-regenerative and immunosuppressive abilities. B-cell involvement in chronic inflammatory and autoimmune pathologies makes them a desirable target for cell-based therapy. Mesenchymal stromal cells are able to regulate B-cell function; although the mechanisms are little known, they imply cell-to-cell contact.

METHODS

We studied the ability of human adipose tissue-derived mesenchymal stromal cells (ASCs) to attract B cells.

RESULTS

We show that ASCs promote B-cell migration through the secretion of chemotactic factors. Inflammatory/innate signals do not modify ASC capacity to mediate B-cell motility and chemotaxis. Analysis of a panel of B cell-related chemokines showed that none of them appeared to be responsible for B-cell motility. Other ASC-secreted factors able to promote cell motility and chemotaxis, such as the cytokine interleukin-8 and prostaglandin E2, did not appear to be implicated.

CONCLUSIONS

We propose that ASC promotion of B-cell migration by undefined secreted factors is crucial for ASC regulation of B-cell responses.

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  • Publisher Full Text
  • Authors+Show Affiliations

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    B Cell Dynamics Laboratory, Department of Immunology and Oncology, Centro Nacional de Biotecnología (CNB)-CSIC, UAM-Campus Cantoblanco, Madrid, Spain.

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    B Cell Dynamics Laboratory, Department of Immunology and Oncology, Centro Nacional de Biotecnología (CNB)-CSIC, UAM-Campus Cantoblanco, Madrid, Spain.

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    TiGenix SAU, Parque Tecnológico de Madrid, Madrid, Spain.

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    TiGenix SAU, Parque Tecnológico de Madrid, Madrid, Spain.

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    TiGenix SAU, Parque Tecnológico de Madrid, Madrid, Spain.

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    TiGenix NV, Leuven, Belgium.

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    TiGenix SAU, Parque Tecnológico de Madrid, Madrid, Spain.

    B Cell Dynamics Laboratory, Department of Immunology and Oncology, Centro Nacional de Biotecnología (CNB)-CSIC, UAM-Campus Cantoblanco, Madrid, Spain. Electronic address: ycarrasco@cnb.csic.es.

    Source

    Cytotherapy 16:12 2014 Dec pg 1692-9

    MeSH

    Adipose Tissue
    B-Lymphocytes
    Cells, Cultured
    Chemotaxis
    Coculture Techniques
    Dinoprostone
    Female
    Humans
    Interleukin-8
    Male
    Mesenchymal Stem Cells

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    25240680

    Citation

    Barrio, Laura, et al. "Human Adipose Tissue-derived Mesenchymal Stromal Cells Promote B-cell Motility and Chemoattraction." Cytotherapy, vol. 16, no. 12, 2014, pp. 1692-9.
    Barrio L, Cuevas VD, Menta R, et al. Human adipose tissue-derived mesenchymal stromal cells promote B-cell motility and chemoattraction. Cytotherapy. 2014;16(12):1692-9.
    Barrio, L., Cuevas, V. D., Menta, R., Mancheño-Corvo, P., delaRosa, O., Dalemans, W., ... Carrasco, Y. R. (2014). Human adipose tissue-derived mesenchymal stromal cells promote B-cell motility and chemoattraction. Cytotherapy, 16(12), pp. 1692-9. doi:10.1016/j.jcyt.2014.07.012.
    Barrio L, et al. Human Adipose Tissue-derived Mesenchymal Stromal Cells Promote B-cell Motility and Chemoattraction. Cytotherapy. 2014;16(12):1692-9. PubMed PMID: 25240680.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Human adipose tissue-derived mesenchymal stromal cells promote B-cell motility and chemoattraction. AU - Barrio,Laura, AU - Cuevas,Victor Delgado, AU - Menta,Ramón, AU - Mancheño-Corvo,Pablo, AU - delaRosa,Olga, AU - Dalemans,Wilfried, AU - Lombardo,Eleuterio, AU - Carrasco,Yolanda R, Y1 - 2014/09/17/ PY - 2014/03/11/received PY - 2014/07/03/revised PY - 2014/07/31/accepted PY - 2014/9/22/entrez PY - 2014/9/23/pubmed PY - 2015/7/30/medline KW - B cells KW - chemotaxis KW - mesenchymal stromal cells KW - migration SP - 1692 EP - 9 JF - Cytotherapy JO - Cytotherapy VL - 16 IS - 12 N2 - BACKGROUND AIMS: Mesenchymal stromal cells hold special interest for cell-based therapy because of their tissue-regenerative and immunosuppressive abilities. B-cell involvement in chronic inflammatory and autoimmune pathologies makes them a desirable target for cell-based therapy. Mesenchymal stromal cells are able to regulate B-cell function; although the mechanisms are little known, they imply cell-to-cell contact. METHODS: We studied the ability of human adipose tissue-derived mesenchymal stromal cells (ASCs) to attract B cells. RESULTS: We show that ASCs promote B-cell migration through the secretion of chemotactic factors. Inflammatory/innate signals do not modify ASC capacity to mediate B-cell motility and chemotaxis. Analysis of a panel of B cell-related chemokines showed that none of them appeared to be responsible for B-cell motility. Other ASC-secreted factors able to promote cell motility and chemotaxis, such as the cytokine interleukin-8 and prostaglandin E2, did not appear to be implicated. CONCLUSIONS: We propose that ASC promotion of B-cell migration by undefined secreted factors is crucial for ASC regulation of B-cell responses. SN - 1477-2566 UR - http://wwww.unboundmedicine.com/medline/citation/25240680/Human_adipose_tissue_derived_mesenchymal_stromal_cells_promote_B_cell_motility_and_chemoattraction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1465-3249(14)00713-0 DB - PRIME DP - Unbound Medicine ER -