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Human adipose tissue-derived mesenchymal stromal cells promote B-cell motility and chemoattraction.
Cytotherapy 2014; 16(12):1692-9C

Abstract

BACKGROUND AIMS

Mesenchymal stromal cells hold special interest for cell-based therapy because of their tissue-regenerative and immunosuppressive abilities. B-cell involvement in chronic inflammatory and autoimmune pathologies makes them a desirable target for cell-based therapy. Mesenchymal stromal cells are able to regulate B-cell function; although the mechanisms are little known, they imply cell-to-cell contact.

METHODS

We studied the ability of human adipose tissue-derived mesenchymal stromal cells (ASCs) to attract B cells.

RESULTS

We show that ASCs promote B-cell migration through the secretion of chemotactic factors. Inflammatory/innate signals do not modify ASC capacity to mediate B-cell motility and chemotaxis. Analysis of a panel of B cell-related chemokines showed that none of them appeared to be responsible for B-cell motility. Other ASC-secreted factors able to promote cell motility and chemotaxis, such as the cytokine interleukin-8 and prostaglandin E2, did not appear to be implicated.

CONCLUSIONS

We propose that ASC promotion of B-cell migration by undefined secreted factors is crucial for ASC regulation of B-cell responses.

Authors+Show Affiliations

B Cell Dynamics Laboratory, Department of Immunology and Oncology, Centro Nacional de Biotecnología (CNB)-CSIC, UAM-Campus Cantoblanco, Madrid, Spain.B Cell Dynamics Laboratory, Department of Immunology and Oncology, Centro Nacional de Biotecnología (CNB)-CSIC, UAM-Campus Cantoblanco, Madrid, Spain.TiGenix SAU, Parque Tecnológico de Madrid, Madrid, Spain.TiGenix SAU, Parque Tecnológico de Madrid, Madrid, Spain.TiGenix SAU, Parque Tecnológico de Madrid, Madrid, Spain.TiGenix NV, Leuven, Belgium.TiGenix SAU, Parque Tecnológico de Madrid, Madrid, Spain.B Cell Dynamics Laboratory, Department of Immunology and Oncology, Centro Nacional de Biotecnología (CNB)-CSIC, UAM-Campus Cantoblanco, Madrid, Spain. Electronic address: ycarrasco@cnb.csic.es.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25240680

Citation

Barrio, Laura, et al. "Human Adipose Tissue-derived Mesenchymal Stromal Cells Promote B-cell Motility and Chemoattraction." Cytotherapy, vol. 16, no. 12, 2014, pp. 1692-9.
Barrio L, Cuevas VD, Menta R, et al. Human adipose tissue-derived mesenchymal stromal cells promote B-cell motility and chemoattraction. Cytotherapy. 2014;16(12):1692-9.
Barrio, L., Cuevas, V. D., Menta, R., Mancheño-Corvo, P., delaRosa, O., Dalemans, W., ... Carrasco, Y. R. (2014). Human adipose tissue-derived mesenchymal stromal cells promote B-cell motility and chemoattraction. Cytotherapy, 16(12), pp. 1692-9. doi:10.1016/j.jcyt.2014.07.012.
Barrio L, et al. Human Adipose Tissue-derived Mesenchymal Stromal Cells Promote B-cell Motility and Chemoattraction. Cytotherapy. 2014;16(12):1692-9. PubMed PMID: 25240680.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human adipose tissue-derived mesenchymal stromal cells promote B-cell motility and chemoattraction. AU - Barrio,Laura, AU - Cuevas,Victor Delgado, AU - Menta,Ramón, AU - Mancheño-Corvo,Pablo, AU - delaRosa,Olga, AU - Dalemans,Wilfried, AU - Lombardo,Eleuterio, AU - Carrasco,Yolanda R, Y1 - 2014/09/17/ PY - 2014/03/11/received PY - 2014/07/03/revised PY - 2014/07/31/accepted PY - 2014/9/22/entrez PY - 2014/9/23/pubmed PY - 2015/7/30/medline KW - B cells KW - chemotaxis KW - mesenchymal stromal cells KW - migration SP - 1692 EP - 9 JF - Cytotherapy JO - Cytotherapy VL - 16 IS - 12 N2 - BACKGROUND AIMS: Mesenchymal stromal cells hold special interest for cell-based therapy because of their tissue-regenerative and immunosuppressive abilities. B-cell involvement in chronic inflammatory and autoimmune pathologies makes them a desirable target for cell-based therapy. Mesenchymal stromal cells are able to regulate B-cell function; although the mechanisms are little known, they imply cell-to-cell contact. METHODS: We studied the ability of human adipose tissue-derived mesenchymal stromal cells (ASCs) to attract B cells. RESULTS: We show that ASCs promote B-cell migration through the secretion of chemotactic factors. Inflammatory/innate signals do not modify ASC capacity to mediate B-cell motility and chemotaxis. Analysis of a panel of B cell-related chemokines showed that none of them appeared to be responsible for B-cell motility. Other ASC-secreted factors able to promote cell motility and chemotaxis, such as the cytokine interleukin-8 and prostaglandin E2, did not appear to be implicated. CONCLUSIONS: We propose that ASC promotion of B-cell migration by undefined secreted factors is crucial for ASC regulation of B-cell responses. SN - 1477-2566 UR - http://wwww.unboundmedicine.com/medline/citation/25240680/Human_adipose_tissue_derived_mesenchymal_stromal_cells_promote_B_cell_motility_and_chemoattraction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1465-3249(14)00713-0 DB - PRIME DP - Unbound Medicine ER -